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[Quantification of isopimpinellin in root of Toddalia asiatica by HPLC]
Xiao-Yan Hao1, Xiao-Hong Cao, Yan Liang
1Department of Pharmacy, Guiyang Medical College, Guiyang 550004, China. haoxiaoyan@vip.163.com
Objective:
To determine the content of isopimpinellin in root of Toddalia asiatica.
Methods:
A HPLC method was set up. Using Hypersil C18 column and methanol-water (70:30) as mobile phase, with the detection wavelength at 306 nm.
Result:
The linear range of isopimpinellin was 0.004 20 approximately 0.420 microg. The average recovery was 99.7% and the RSD 2.8%.
Conclusion:
The method is simple and accurate, with good reproducibility, and can be used as a quantitative analysis method for isopimpinellin.
