Related Experiment Video
Updated: Jul 9, 2026
06:50
Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers
Published on: February 29, 2012
Rational principles for modulating fluorescence properties of fluorescein
Tasuku Ueno1, Yasuteru Urano, Ken-Ichi Setsukinai
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Journal of the American Chemical Society
|October 28, 2004
Summary
Researchers developed a new method to control fluorescein dye properties by modifying its benzene part. This strategy enables the rational design of novel fluorescence probes for specific targets.
Area of Science:
- Organic Chemistry
- Photochemistry
- Materials Science
Background:
- Developing targeted fluorescence probes is crucial for molecular detection.
- Existing methods for modulating fluorescence properties often lack generality.
- Fluorescein is a widely used fluorophore with potential for functionalization.
Purpose of the Study:
- To present a general principle for modulating fluorescein fluorescence properties.
- To establish a rational design strategy for novel fluorescence probes.
- To investigate the relationship between molecular structure and fluorescence modulation.
Main Methods:
- Dividing fluorescein into a fluorophore (xanthene) and a fluorescence-controlling moiety (benzene).
- Utilizing a photoinduced electron transfer (PeT) process, specifically donor-excited PeT (d-PeT).
- Synthesizing fluorescein derivatives with fine-tuned reduction potentials in the benzene moiety via electron-withdrawing groups.
Main Results:
- Demonstrated that fluorescein's fluorescence properties are modulated by the reduction potential of the benzene moiety.
- Confirmed the d-PeT mechanism's role in fluorescence modulation.
- Established a structure-property relationship for fluorescein derivatives.
Conclusions:
- The proposed principle offers a practical approach for designing functional fluorescence probes.
- Fine-tuning the benzene moiety's reduction potential effectively modulates fluorescein's fluorescence.
- This work provides a foundation for developing advanced fluorescent sensing technologies.
Related Concept Videos
02:00Photoreceptors and Plant Responses to Light
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
01:22Photoreceptors and Visual Pathways
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...
01:16Biasing of P-N Junction
The operation of a p-n junction diode involves various biasing conditions, including forward bias, reverse bias, and equilibrium.
In equilibrium, no external voltage is applied across the p-n junction. The depletion region is formed at the junction interface due to the diffusion of carriers, which leaves behind charged dopants, acceptors on the p-side, and donors on the n-side. These immobile charges create an electric field that prevents further diffusion of carriers. The related energy band...
In equilibrium, no external voltage is applied across the p-n junction. The depletion region is formed at the junction interface due to the diffusion of carriers, which leaves behind charged dopants, acceptors on the p-side, and donors on the n-side. These immobile charges create an electric field that prevents further diffusion of carriers. The related energy band...
01:21Time and frequency -Domain Interpretation of Phase-lag Control
Phase-lag controllers are widely used in control systems to improve stability and reduce steady-state errors. A dimmer switch controlling the brightness of a light bulb serves as a practical example of phase-lag control, gradually adjusting the bulb's brightness. Mathematically, phase-lag control or low-pass filtering is represented when the factor 'a' is less than 1.
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any finite,...
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any finite,...