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Viral detection.

Feng Wang-Johanning1, Gary L Johanning

  • 1Department of Veterinary Sciences, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2004
PubMed
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This chapter details DNA virus detection using amplification methods like real-time PCR. It focuses on quantifying HPV16 oncogenes in human tissues for enhanced diagnostics.

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Accurate detection and quantification of DNA viruses are crucial for diagnosing and managing viral infections.
  • Human Papillomavirus (HPV), particularly HPV16, is linked to oncogenesis through specific oncoproteins.
  • Existing diagnostic methods require optimization for sensitivity and specificity.

Purpose of the Study:

  • To provide a comprehensive overview of DNA virus detection techniques.
  • To detail the amplification and quantification of HPV16 E6 and E7 oncoproteins.
  • To discuss data analysis for real-time PCR-based viral detection.

Main Methods:

  • Focus on amplification reactions: Polymerase Chain Reaction (PCR), Reverse Transcription-PCR (RT-PCR), real-time PCR, and real-time RT-PCR.

Related Experiment Videos

  • Detailed description of primer and probe design for amplifying HPV16 E6 and E7 oncogenes.
  • Presentation of quantification techniques for oncogenes in human tissue specimens.

    • Main Results:

    • Methodologies for sensitive detection and quantification of viral DNA are presented.
    • Specific primers and probes for HPV16 oncogene amplification are described.
    • Data analysis strategies for real-time PCR results are discussed.

    Conclusions:

    • Amplification-based methods, especially real-time PCR, offer robust detection of DNA viruses.
    • Quantification of viral oncogenes like HPV16 E6/E7 is feasible in clinical specimens.
    • The discussed techniques contribute to improved viral diagnostics and research.