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Published on: January 12, 2024
DNA affinity purification of Epstein-Barr virus OriP-binding proteins
Constandache Atanasiu1, Larissa Lezina, Paul M Lieberman
1The Wistar Institute, Philadelphia, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2004
Abstract:
DNA affinity purification has been used to identify cellular and viral proteins associated with the Epstein-Barr virus origin of plasmid DNA replication. This approach allows for a one- or two-step purification scheme of high-affinity DNA binding proteins from crude nuclear extracts. Additionally, this approach may be useful for isolation of proteins that are found in the insoluble fractions of the nuclear matrix or scaffold.

