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Genotoxicity evaluation of Isaria sinclairii (ISE) extract
Mi Young Ahn1, Kang Sun Ryu, Sang Duck Jee
1Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea.
Journal of Toxicology and Environmental Health. Part A
|October 30, 2004
Summary
Isaria sinclairii, a traditional Chinese medicine, was evaluated for mutagenic potential. Short-term genotoxicity tests, including the Ames, chromosome aberration, and micronuclei tests, showed no mutagenic effects in vitro or in vivo.
Area of Science:
- Pharmacology and Toxicology
- Natural Product Chemistry
- Genetics and Molecular Biology
Background:
- Isaria sinclairii is a fungus used in traditional Chinese medicine.
- Its safety profile, particularly regarding mutagenicity, requires thorough scientific evaluation.
- Understanding the genotoxic potential is crucial for its medicinal applications.
Purpose of the Study:
- To assess the mutagenic potential of Isaria sinclairii extract (ISE).
- To evaluate ISE using a battery of standard genotoxicity assays.
- To determine the safety of ISE for potential therapeutic use.
Main Methods:
- Ames test using Salmonella typhimurium strains (TA98, TA100, TA1535, TA1537).
- In vitro chromosome aberration (CA) test on Chinese hamster ovary (CHO) cells.
- In vivo micronuclei (MN) test in male ICR mice.
Main Results:
- No mutagenic response observed in the Ames test, with or without metabolic activation (S9 mix).
- No significant induction of chromosomal aberrations in CHO cells treated with ISE.
- No significant increase in micronucleated polychromatic erythrocytes in mice administered ISE at various doses.
Conclusions:
- Isaria sinclairii extract demonstrates no mutagenic potential in the conducted in vitro and in vivo genotoxicity tests.
- The findings support the safety of Isaria sinclairii for medicinal use.
- Further toxicological studies may be warranted to fully characterize its safety profile.