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Updated: Aug 21, 2026
10:28
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Published on: August 17, 2019
A simple visual assay based on small molecule hydrogels for detecting inhibitors of enzymes
1Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Chemical Communications (Cambridge, England)
|October 30, 2004
Abstract:
Here we report a simple approach to develop assays based on the hydrogelation of small molecules for quick detecting inhibitors of enzymes.
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01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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01:30Enzyme Inhibition
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.