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Tumor cell-specific transcription of a murine histocompatibility class Ib Q5 gene
Katsuo Noguchi1, Eri Kuwada, Shigenori Goto
1Institute of Molecular Immunology, Medinet, Tamagawadai, Setagaya-ku, Tokyo 158-0096 and 2Seta Clinic, 4-20-18 Seta, Setagayaku, Tokyo 158-0095, Japan. noguchi@j-immunother.com
Background:
We previously reported that the Q5 gene product (Q5 antigen) was expressed on the surface of various tumor cells derived from H-2k (Qa-2-) mice. The Q5 antigen has tumor-protective antigenicity in the syngeneic mice.
Materials And Methods:
Transcripts of the Qa region genes were analyzed by the RT-PCR method. Cell fractionation was performed with the MACS method and the phenotypes were estimated by flow cytometry analysis.
Results:
Transcripts of Q5 were produced by all tumor cell lines. The Q5 transcription was detected only in thymocytes and PBMCs of H-2k (AKR and C3H/He) mice. The phenotype of the PBMCs in which Q5 transcription takes place seems to be, at least partly, CD3-48 cells that might be related to CD3-4-8- spontaneous thymoma cells derived from an H-2k mouse.
Conclusion:
The expression of Q5 in tumor cells in general is a result of a change of transcriptional regulation associated with malignant transformation.
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