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Related Experiment Videos

Porous polymer monolith assisted electrospray.

Terry Koerner1, Kiera Turck, Laurie Brown

  • 1Department of Chemistry, Queen's University, Kingston, Ontario, Canada K7L 3N6.

Analytical Chemistry
|November 2, 2004
PubMed
Summary

This study introduces a novel porous polymer monolith (PPM) for fabricating robust nanospray emitters. This innovation overcomes limitations of conventional methods, enabling stable electrospray ionization mass spectrometry (ESI-MS) with improved performance.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Separation Science

Background:

  • Coupling low-flow analytical separation instruments with electrospray ionization mass spectrometry (ESI-MS) creates powerful analytical tools.
  • Conventional nanospray emitters face challenges like poor robustness, clogging, complex fabrication, and limited flow rate compatibility.

Purpose of the Study:

  • To develop a robust and reliable nanospray emitter for ESI-MS.
  • To overcome the limitations associated with conventional nanospray emitter fabrication and performance.

Main Methods:

  • Fabrication of robust nanospray emitters using a photopatterned porous polymer monolith (PPM) at the fused-silica capillary exit.
  • Characterization of electrospray stability and performance across a range of flow rates (50-1000 nL/min).

Related Experiment Videos

  • Evaluation of PPM properties, including hydrophobicity and multiple flow paths, for improved emitter function.

    • Main Results:

    • Stable electrosprays were achieved from capillaries without tapering, using PPM emitters at flow rates from 50-1000 nL/min.
    • The PPM facilitated stable Taylor cone formation and minimized clogging, while its hydrophobic nature reduced band broadening.
    • Total ion current traces showed high stability (3-8% deviation), and mass spectra exhibited excellent signal-to-noise ratios for femtomole quantities.

    Conclusions:

    • Porous polymer monoliths provide a robust and effective method for fabricating advanced nanospray emitters.
    • This PPM-assisted approach enhances ESI-MS performance, offering stability and sensitivity for low-flow analytical separations.
    • The developed emitters are compatible with a wider range of flow rates and exhibit improved resistance to clogging and band broadening.