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Related Experiment Videos

The CorA Mg2+ transporter does not transport Fe2+.

Krisztina M Papp1, Michael E Maguire

  • 1Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, OH 44106-4965, USA.

Journal of Bacteriology
|November 2, 2004
PubMed
Summary

The CorA protein, essential for magnesium (Mg2+) transport in bacteria, does not transport iron (Fe2+). Research indicates that mutations in corA do not alter bacterial sensitivity to iron toxicity, suggesting an indirect relationship.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • CorA is the primary magnesium (Mg2+) transporter in many bacteria and archaea.
  • A previous study suggested CorA might mediate iron (Fe2+) influx, causing resistance to Fe2+-mediated toxicity.

Purpose of the Study:

  • To investigate the direct role of CorA in Fe2+ transport and its contribution to Fe2+ toxicity.
  • To clarify the relationship between CorA function and iron homeostasis in *Salmonella enterica* serovar Typhimurium.

Main Methods:

  • Direct Fe2+ transport assays were performed using wild-type and *corA* mutant *Salmonella enterica* serovar Typhimurium strains.
  • Inhibition assays examined the effect of Fe2+ on Ni2+ transport by CorA.
  • Differential toxicity assays assessed sensitivity to excess Fe2+ between wild-type and *corA* cells.

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Main Results:

  • CorA was confirmed not to transport Fe2+.
  • Fe2+ did not significantly inhibit Ni2+ transport mediated by CorA.
  • Mg2+ weakly inhibited Fe2+ uptake, independent of CorA.
  • No significant difference in sensitivity to Fe2+ toxicity was observed between wild-type and *corA* mutant strains.

Conclusions:

  • CorA does not function as an Fe2+ transporter.
  • The previously reported resistance to Fe2+ toxicity in *corA* mutants is not due to altered Fe2+ transport by CorA.
  • Any link between *corA* and iron toxicity is indirect and requires further investigation.