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Updated: Aug 21, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
[Utilization of H2O2 as the oxygen source by bacteria of the genera Pseudomonas and Rhodococcus]
Abstract:
The growth of bacteria of the genera Pseudomonas and Rhodococcus in the presence of hydrogen peroxide as the sole source of oxygen was studied. The toxic effect of H2O2 in the concentration range of 100-200 microg/ml was shown to extend the lag phase by 2 to 3 days. Apart from the peroxide toxicity, the bacterial growth was inhibited by the toxic effect of dissolved oxygen in concentrations over 100 microg O2/ml; in the presence of a liquid hydrocarbon phase, this effect was alleviated. Under decreased partial pressure of oxygen in the presence of hydrocarbons (12-15 vol %), the culture growth was initiated at high initial concentrations of H2O2 (300 microg/ml). When hydrogen peroxide concentrations exceeded 320 microg/ml, no growth occurred, no matter how much hydrocarbon was added.
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