Related Experiment Video
Updated: Aug 21, 2026
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
Published on: April 25, 2016
[Tricholoma equestre--animal toxicity study]
Zygmunt Chodorowski1, Małgorzata Sznitowska, Marek Wiśniewski
1I Klinika Chorób Wewnetrznych i Ostrych Zatruć, Akademii Medycznej w Gdańsku. jsanand@amedec.amg.gda.pl
Unlabelled:
Animal toxicity study of Tricholoma equestre mushrooms stored for 12 months at (-)20 degrees C was performed using 30 male BALB/c mice. Three groups of 5 mice each were given suspension of T. equestre powder in water, boiled aqueous extract and chloroform-methanol extract dissolved in Miglyol 812 by gavage for three consecutive days. Mice in control groups were given water, Miglyol 812 and p-phenylenediamine (CAS 106-50-3). Creatine kinase activity was determined in serum collected 72 hours after the final dose. Mean activity of serum creatine kinase in mice treated with T. equestre powder, aqueous extract, chloroform-methanol extract and Miglyol 812 were 157 +/- 93, 129 +/- 30, 96 +/- 38, 111 +/- 66 U/L respectively and did not differ significantly from mean activity in mice which were given water (107 +/- 38 U/L). Mean serum creatine kinase activity in p-phenylenediamine group (265 +/- 63 U/L) was significantly higher than in group treated with water (p<0.01).
Conclusion:
Extracts of Tricholoma equestre mushrooms stored for 12 months at (-)20 degrees C did not cause rhabdomyolysis in male BALB/c mice.
Insights
This study found that Tricholoma equestre mushroom extracts, stored for 12 months at -20°C, did not cause muscle damage (rhabdomyolysis) in mice. The creatine kinase levels remained normal across all tested mushroom extract groups.
Area of Science:
- Mycology
- Toxicology
- Biochemistry
Context:
- Tricholoma equestre, a widely consumed mushroom, has been associated with potential toxicity.
- Understanding the safety of long-term stored mushroom extracts is crucial for culinary and medicinal use.
Purpose:
- To evaluate the potential toxicity of stored Tricholoma equestre mushroom extracts.
- To assess the impact of mushroom extracts on muscle integrity by measuring serum creatine kinase activity.
Summary:
- Male BALB/c mice were administered various Tricholoma equestre extracts (powder, aqueous, chloroform-methanol) and controls for three days.
- Serum creatine kinase (CK) levels were measured 72 hours post-administration.
- No significant elevation in CK levels was observed in mice treated with mushroom extracts compared to the water control group.
Impact:
- The findings suggest that Tricholoma equestre mushroom extracts stored under specified conditions do not induce rhabdomyolysis in mice.
- This research contributes to the safety assessment of edible mushrooms and their processed forms.
Related Concept Videos
01:23Toxicity Testing in Animals
01:21Antiprotozoal Agents