Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thrombus-induced endothelial dysfunction: Hemoglobin and fibrin decrease nitric oxide bioactivity without altering

Michael R Davis1, Colleen M Fitzpatrick, Patricia M Dixon

  • 1Division of Vascular Surgery, Wilford Hall Medical Center, Lackland AFB, Texas, USA.

The Journal of Surgical Research
|November 4, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Forecasting Patient-Specific Abdominal Aortic Aneurysm Geometry with Mixed-Effects Models.

Diagnostics (Basel, Switzerland)·2026
Same author

From battlefield to bedside: How military surgeons advanced pediatric critical care transport and ECMO.

Seminars in pediatric surgery·2026
Same author

Major Adverse Limb Events and Death After Successful Endovascular Revascularization: BEST-CLI Trial.

Journal of the Society for Cardiovascular Angiography & Interventions·2026
Same author

Geometry quantification for growth assessment of abdominal aortic aneurysms under surveillance.

Scientific reports·2026
Same author

An Integrated Framework for Automated Image Segmentation and Personalized Wall Stress Estimation of Abdominal Aortic Aneurysms.

Bioengineering (Basel, Switzerland)·2026
Same author

The Climate Health Curriculum Tool (CHCT): an assessment framework for integrating climate health education in U.S. undergraduate medical curricula.

BMC medical education·2026

Free hemoglobin and fibrin in thrombi reduce nitric oxide (NO) bioactivity, impacting endothelial function. However, thrombus components do not permanently alter endothelial nitric oxide synthase (eNOS) expression or activity.

Area of Science:

  • Cardiovascular Biology
  • Endothelial Cell Function
  • Thrombosis Research

Background:

  • Arterial thrombosis is a significant contributor to cardiovascular disease, often linked to endothelial dysfunction.
  • Understanding the specific thrombus components that trigger endothelial dysfunction is crucial for developing targeted therapies.

Purpose of the Study:

  • To identify which thrombus components induce endothelial dysfunction.
  • To investigate the impact of these components on endothelial nitric oxide synthase (eNOS) activity and expression.

Main Methods:

  • Human aortic endothelial cells were exposed to various thrombus components: erythrocytes (RBCs), platelets, hemoglobin, thrombin, and fibrin.
  • Nitric oxide (NO) levels, cellular integrity (MTS metabolism), eNOS mRNA expression, and eNOS activity were measured.

Related Experiment Videos

Main Results:

  • Free hemoglobin and fibrin significantly reduced NO levels and cellular integrity.
  • Thrombin demonstrated a concentration-dependent effect on NO production, inhibiting at low doses and stimulating at high doses.
  • RBCs and platelets did not affect NO production or cellular integrity, and eNOS expression and activity remained unchanged across all tested thrombus components.

Conclusions:

  • Extracellular thrombus components, specifically free hemoglobin and fibrin, impair endothelial NO bioactivity.
  • Thrombin modulates NO levels concentration-dependently.
  • Thrombus exposure does not lead to lasting alterations in endothelial eNOS expression or activity, suggesting reversible endothelial dysfunction.