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Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus

Wang Jing1, Sun Shuhan, Hu Zhenlin

  • 1Department of Pathophysiology, Hebei Medical University, Shijiazhuang, China.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|November 4, 2004
PubMed

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...