[Assessement of an automated homogeneous liposome-based immunoassay system for total complement activity]

The Immunology department of Garcia de Orta Hospital, has evaluated a new automated homogeneous liposome-based immunoassay (L.I.A.), for measuring total complement activity, which has become recently commercially available from WAKO (Osaka, Japan), (CH50 WAKO). Serum samples from 100 healthy voluntary donors were assayed, with liposome-based assay. The reference values in our adult population concerning CH50 were established. Within-run and between-run precision, accuracy, carry-over and the linearity of the new method were also assessed.