Related Experiment Videos
High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging
J Requejo-Isidro1, J McGinty, I Munro
1Department of Physics, Division of Investigative Sciences and Department of Bioengineering, Imperial College London, London, UK. j.requejo@imperial.ac.uk
Optics Letters
|November 5, 2004
Summary
We developed a high-speed fluorescence-lifetime imaging (FLIM) system capable of 29 frames/s. This technology enables advanced FLIM multiwell plate readers and portable endoscopic imaging systems.
Area of Science:
- Biophotonics
- Medical Imaging
Background:
- Fluorescence-lifetime imaging (FLIM) is a powerful technique for analyzing biological samples.
- High-speed imaging is crucial for dynamic biological processes and clinical applications.
Purpose of the Study:
- To develop a novel high-speed wide-field fluorescence-lifetime imaging (FLIM) system.
- To demonstrate the system's capabilities in multiwell plate reading and endoscopic imaging.
Main Methods:
- Implementation of a wide-field FLIM system with advanced detection capabilities.
- Integration of the FLIM system with a multiwell plate reader.
- Development of a portable FLIM endoscopic probe.
Main Results:
- Achieved high-speed FLIM image acquisition at rates up to 29 frames per second.
- Successfully demonstrated a FLIM multiwell plate reader.
- Showcased a functional, potentially portable FLIM endoscopic system operating at 355-nm excitation.
Conclusions:
- The developed high-speed FLIM system significantly advances imaging capabilities.
- The demonstrated applications highlight the system's versatility for research and potential clinical use.