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Measuring the Kinetics of mRNA Transcription in Single Living Cells
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Measuring the Kinetics of mRNA Transcription in Single Living Cells

Published on: August 25, 2011

Real-time monitoring of in vitro transcriptional RNA by using fluorescence correlation spectroscopy

Yasutomo Nomura1, Masataka Kinjo

  • 1Department of Bio-System Engineering, Faculty of Engineering, Yamagata University, Yonezawa 992-8510, Japan. ynomura@yz.yamagata-u.ac.jp

Chembiochem : a European Journal of Chemical Biology
|November 5, 2004
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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