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Fluoride flux in the rabbit CCD: a pH-dependent event
A J Rouch1, G M Whitford, H T Campbell
1Department of Physiology and Endocrinology, School of Medicine, Medical College of Georgia, Augusta.
Kidney International
|February 1, 1992
Summary
Luminal pH is the main factor controlling fluoride flux in rabbit kidneys. Lowering pH significantly increases fluoride transport, independent of water flux or chloride pathways.
Area of Science:
- Nephrology
- Renal Physiology
- Ion Transport
Background:
- Fluoride transport in the kidney is not fully understood.
- The cortical collecting duct (CCD) plays a role in regulating urine composition.
- Investigating fluoride flux (JF) determinants is crucial for understanding renal handling of fluoride.
Purpose of the Study:
- To identify the primary factors influencing fluoride flux (JF) in the isolated rabbit cortical collecting duct (CCD).
- To determine if water flux, pH, specific inhibitors, or chloride substitution affect JF.
- To elucidate the transport pathway for fluoride in the rabbit CCD.
Main Methods:
- Isolated rabbit cortical collecting duct (CCD) perfused with fluoride-containing solution.
- Manipulation of luminal pH and measurement of fluoride flux (JF).
- Assessment of effects of osmotic water flux, acetazolamide, DIDS, and chloride-gluconate substitution on JF.
Main Results:
- Luminal pH significantly impacts JF; decreasing pH increases fluoride flux.
- Osmotic water flux, acetazolamide, DIDS, and chloride substitution did not significantly alter JF.
- No correlation was observed between JF and transepithelial voltage.
Conclusions:
- Luminal pH is the principal determinant of fluoride flux in the rabbit CCD.
- Fluoride transport in the rabbit CCD does not appear to involve chloride-mediated or DIDS-inhibitory pathways.
