Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quasimonomorphic mononucleotide repeats for high-level microsatellite instability analysis.

Olivier Buhard1, Nirosha Suraweera, Aude Lectard

  • 1INSERM U434, CEPH, Paris, France.

Disease Markers
|November 6, 2004
PubMed
Summary

Microsatellite instability (MSI) analysis is crucial for cancer detection. This study compares mononucleotide and dinucleotide repeats for more accurate MSI tumor identification, addressing limitations of previous methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond p53 status: the continued importance of histopathologic features for predicting lymph node metastasis in endometrial cancer.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2026
Same author

Genomics-based assessment of the geographic origin of spongy moths (Lymantria dispar) intercepted during vessel inspections, using SpongySeq, an amplicon sequencing panel.

BMC genomics·2026
Same author

Lifestyles shape genome size and gene content in fungal pathogens.

eLife·2025
Same author

MSI expresso: a software for determining MSI status and detecting MSI-related transcription events from RNA sequencing data.

Frontiers in genetics·2025
Same author

Chromatin Organization Governs Transcriptional Response and Plasticity of Cancer Stem Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Tracking the North American Asian Longhorned Beetle Invasion With Genomics.

Evolutionary applications·2024

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Microsatellite instability (MSI) analysis is vital for detecting sporadic tumors and Hereditary Non-Polyposis Colorectal Cancer (HNPCC).
  • Previous MSI detection methods faced challenges due to a lack of consensus markers, leading to conflicting data.
  • Bethesda consensus meetings aimed to standardize MSI detection, proposing marker sets.

Purpose of the Study:

  • To compare the efficacy of mononucleotide versus dinucleotide repeats in determining microsatellite instability.
  • To evaluate proposed changes in MSI marker panels following the second Bethesda consensus meeting.
  • To highlight the advantages of a novel pentaplex MSI screening test using quasimonomorphic mononucleotide repeats.

Main Methods:

  • Review and comparison of existing data on mononucleotide and dinucleotide repeat markers for MSI analysis.

Related Experiment Videos

  • Discussion of the limitations of the 1998 Bethesda microsatellite panel.
  • Presentation of a proposed pentaplex MSI screening test utilizing quasimonomorphic mononucleotide repeats.

    • Main Results:

    • The 1998 microsatellite panel may inaccurately classify high-level and low-level MSI tumors.
    • Mononucleotide repeats are suggested as a superior alternative to dinucleotide repeats for MSI determination.
    • A pentaplex screening test with mononucleotide repeats offers a potentially more accurate MSI detection method.

    Conclusions:

    • Mononucleotide repeats offer advantages over dinucleotide repeats for accurate microsatellite instability analysis.
    • Refined MSI marker panels, focusing on mononucleotide repeats, are essential for improved cancer diagnostics.
    • The proposed pentaplex MSI screening test warrants further investigation for clinical application.