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Ex vivo cutaneous absorption assessmentof a stabilized ascorbic acid formulation using a microdialysis system.
1Laboratoire d'Ingénierie et Biologie Cutanées et Institut Fédératif de Recherche, Besançon, France.
Skin Pharmacology and Physiology
|November 6, 2004
Summary
Topical application of an 8% ascorbic acid formulation rapidly penetrates the skin. This antioxidant provides sustained photoprotection without degradation, offering a promising strategy against UV damage.
Area of Science:
- Dermatology
- Photoprotection
- Cosmetic Science
Background:
- Ultraviolet (UV) light generates reactive oxygen species, leading to skin photodamage and photoaging.
- Antioxidants are crucial for mitigating UV-induced skin damage.
Purpose of the Study:
- To evaluate the ex vivo skin penetration and degradation of ascorbic acid from a novel 8% topical formulation.
- To assess the potential of this formulation for photoprotection.
Main Methods:
- Human skin fragments were treated with an 8% ascorbic acid formulation.
- Microdialysis was employed to collect ascorbic acid and its metabolites from the skin.
- Gas chromatography-mass spectrometry was used for quantitative analysis.
Main Results:
- Topical application resulted in significantly elevated ascorbic acid levels in dermal tissue.
- Sustained high concentrations of ascorbic acid were maintained with repeated application every 8 hours.
- No evidence of ascorbic acid degradation was observed in the skin.
Conclusions:
- Ascorbic acid demonstrates rapid penetration into the skin following topical application.
- The formulation establishes a persistent dermal reservoir of ascorbic acid.
- This sustained antioxidant presence represents an effective photoprotection strategy.