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AF1q, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron-like cells
Hsingchi J Lin1, Kara M Shaffer, Zairen Sun
1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA. jlin@cbmse.nrl.navy.mil
Brain Research. Molecular Brain Research
|November 9, 2004
Abstract:
Three cell groups, neural stem/progenitor cells (NS/PCs) dissociated from the embryonic day 11 (E11) rodent cerebral cortex, expanded NS/PC cultures, and cultured neurons from E15, were used to conduct a genomic study with differential display (DD). The mouse Af1q, homologue of human AF1q, was found to be significantly up-regulated during the neuronal production from NS/PCs. The ectopic expression of human AF1q triggered the expression of the neuronal marker TuJ1 in non-neuronal human embryonic kidney (HEK) cells.