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Updated: Aug 21, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
High resolution electrospray and electrospray tandem mass spectra of rotenone and its isoxazoline cycloadducts
Massimiliano Cordaro1, Leonardo Di Donna, Giovanni Grassi
1Dipartimento di Chimica Organica e Biologica, Università, Vill. S. Agata, I-98166 Messina, Italy.
Abstract:
An evaluation of the gas-phase ion chemistry of rotenone (1) by electrospray ionisation (ESI) mass spectrometry (MS) and tandem mass spectrometry (MS2) is presented, aiming at providing tools for its determination in natural and biological matrices. The behaviour of its cycloadducts with benzonitrile-N-oxide (2) and 2,4,6-trimethylbenzonitrile-N-oxide (3) was also evaluated and the MS data thus obtained have provided evidence into the mechanism of formation of the key product ion at m/z 192 which can be considered a marker in the MS and MS2 spectra of rotenone and its derivatives.
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