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Mitomycin concentration in rabbit and human ocular tissues after topical administration
K Kawase1, H Matsushita, T Yamamoto
1Department of Ophthalmology, Gifu University School of Medicine, Japan.
Ophthalmology
|February 1, 1992
Summary
Mitomycin C (MMC) rapidly clears from ocular tissues after subconjunctival injection. Irrigating the eye surface significantly reduces MMC concentration in the conjunctiva and sclera, aiding in drug concentration management.
Area of Science:
- Ophthalmology
- Pharmacokinetics
- Drug Delivery
Background:
- Mitomycin C (MMC) is used in ophthalmic procedures.
- Understanding MMC's concentration and clearance in ocular tissues is crucial for optimizing its therapeutic use and minimizing side effects.
Purpose of the Study:
- To measure mitomycin C (MMC) concentrations in ocular tissues.
- To determine the pharmacokinetic profile of MMC in rabbit ocular tissues after subconjunctival injection.
- To evaluate the effect of ocular surface irrigation on MMC concentrations.
Main Methods:
- High-performance liquid chromatography (HPLC) was used to quantify MMC concentrations.
- Rabbit eyes were used to study MMC pharmacokinetics after subconjunctival injection.
- Human trabeculectomy specimens were analyzed for MMC levels post-application and irrigation.
Main Results:
- MMC exhibited rapid elimination from conjunctiva (half-life: 0.18–0.30 hours) and sclera (half-life: 0.20–0.45 hours).
- Ocular surface irrigation with saline significantly reduced initial MMC concentrations in sclera (by 5-fold) and conjunctiva (by 15-fold).
- MMC concentrations in human trabeculectomy specimens were comparable to rabbit scleral concentrations after irrigation.
Conclusions:
- Mitomycin C is rapidly cleared from ocular tissues.
- Ocular surface irrigation is an effective method to reduce residual MMC concentrations in ocular tissues.
- These findings have implications for optimizing MMC dosing and application protocols in ophthalmic surgery.