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Oxidative stress plays a role in diabetes-induced bladder dysfunction in a rat model
1Department of Urology, McGill University, Montreal, Quebec, Canada.
Urology
|November 10, 2004
Summary
Diabetes induction causes oxidative stress in rat bladders, damaging smooth muscle cells. This oxidative damage may contribute to diabetic cystopathy, a complication of diabetes.
Area of Science:
- Urology
- Endocrinology
- Pathology
Background:
- Oxidative stress is implicated in diabetes complications.
- The role of oxidative stress in diabetic cystopathy remains unstudied.
Purpose of the Study:
- To evaluate bladder oxidative status 8 weeks after diabetes induction in rats.
- To investigate the link between oxidative stress and diabetic cystopathy.
Main Methods:
- Streptozotocin-induced diabetic rats were compared to control and diuretic control groups.
- Bladder antioxidant enzyme activity, lipid peroxidation, inducible nitric oxide synthase, and apoptosis were measured.
Main Results:
- Diabetic rat bladders showed reduced catalase activity and increased lipid peroxidation.
- Increased inducible nitric oxide synthase and apoptotic cells were observed in diabetic bladder smooth muscle.
- Oxidative stress was confirmed in diabetic bladders, independent of diuresis.
Conclusions:
- Oxidative stress occurs in the bladders of streptozotocin-diabetic rats.
- Oxidative damage to smooth muscle cells may contribute to diabetic cystopathy.