Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Candida biofilm resistance.

Pranab K Mukherjee1, Jyotsna Chandra

  • 1Department of Dermatology, Center for Medical Mycology, Case Western Reserve University, 11100 Euclid Avenue, LKS-5028 Cleveland, OH 44106-5028, USA. pkm@cwru.edu

Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy
|November 10, 2004
PubMed
Summary

Device biofilms, often caused by Candida species, show high resistance to antifungals. Mechanisms include drug efflux pumps and altered membrane sterols, contributing to persistent fungal infections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HSD3B1 links ileal steroid metabolism to bile acid regulation in patients with prostate cancer.

The Journal of clinical investigation·2026
Same author

A metabolic constraint in de novo NAD+ synthesis drives mucosal inflammation in IBD.

Journal of Crohn's & colitis·2026
Same author

Elovanoid neuroprotection targets cell transcriptomics and proteomics to sustain synaptic integrity after brain injury.

Communications biology·2026
Same author

Transglutaminase 2 in chronic inflammation and fibrosis - a potential novel therapy for stricturing Crohn's disease.

Expert review of clinical immunology·2025
Same author

The functional role of the extracellular matrix in inflammatory bowel disease associated gut fibrosis.

Matrix biology : journal of the International Society for Matrix Biology·2025
Same author

Protocol for the decellularization of human intestinal tissue from patients with inflammatory bowel disease.

STAR protocols·2025

Area of Science:

  • Mycology
  • Infectious Diseases
  • Biotechnology

Background:

  • Device-related infections are a significant challenge in healthcare settings.
  • Biofilms formed by microbial pathogens on medical devices contribute to persistent infections.
  • Candida species are a common cause of fungal device-related infections, exhibiting high antimicrobial resistance.

Purpose of the Study:

  • To investigate the mechanisms underlying the enhanced antifungal resistance of Candida biofilms.
  • To identify factors contributing to the persistence of fungal biofilms despite antifungal therapy.

Main Methods:

  • Analysis of gene expression related to drug efflux pumps during early biofilm formation.
  • Assessment of membrane sterol composition in Candida biofilms.

Related Experiment Videos

  • Evaluation of the role of extracellular matrix (ECM) in drug resistance and diffusion.

    • Main Results:

    • Candida biofilms demonstrate significantly enhanced resistance to most antifungal agents, with exceptions including echinocandins and lipid formulations of amphotericin B.
    • Upregulation of drug efflux pumps and alterations in membrane sterol composition are key contributors to azole resistance in these biofilms.
    • Metabolic dormancy and the extracellular matrix (ECM) do not appear to be primary drivers of resistance, though ECM can impede drug diffusion in mixed-species biofilms.

    Conclusions:

    • Multifactorial resistance mechanisms in fungal biofilms provide broad-spectrum defense against various antifungal agents.
    • Understanding these mechanisms is crucial for developing effective strategies against persistent Candida biofilm infections.
    • These resistance strategies in fungal biofilms share common themes with those observed in other microbial biofilms.