Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional implications of interleukin-1 beta based on the three-dimensional structure.

B Veerapandian1, G L Gilliland, R Raag

  • 1Center for Advanced Research in Biotechnology, University of Maryland, Rockville.

Proteins
|January 1, 1992
PubMed
Summary

The molecular structure of interleukin-1 beta (IL-1) was determined, revealing a beta-barrel core crucial for its function. This structure suggests a binding surface for IL-1 receptor interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Macromolecular Crystallography and Structural Biology Databases at NIST.

Journal of research of the National Institute of Standards and Technology·2016
Same author

Sickness absence in student nursing assistants following a preventive intervention programme.

Occupational medicine (Oxford, England)·2010
Same author

Low-frequency dynamics of Caldariomyces fumago chloroperoxidase probed by femtosecond coherence spectroscopy.

Biochemistry·2008
Same author

Structure of monellin refined to 2.3 a resolution in the orthorhombic crystal form.

Acta crystallographica. Section D, Biological crystallography·2004
Same author

Mesopone cytochrome c peroxidase: functional model of heme oxygenated oxidases.

Journal of inorganic biochemistry·2002
Same author

Crystal structure of dephospho-coenzyme A kinase from Haemophilus influenzae.

Journal of structural biology·2002

Area of Science:

  • Biochemistry
  • Structural Biology
  • Immunology

Background:

  • Interleukin-1 beta (IL-1) is a cytokine involved in various disease processes.
  • Understanding IL-1's molecular structure is key to elucidating its function.

Purpose of the Study:

  • To determine the high-resolution molecular structure of interleukin-1 beta.
  • To investigate the functional implications of its three-dimensional structure.

Main Methods:

  • X-ray crystallography at 2.0 A resolution.
  • Refinement to a crystallographic R-factor of 0.19.
  • Site-directed mutagenesis, biochemical, and immunological studies.

Main Results:

  • The molecular structure of IL-1 consists of 12 antiparallel beta-strands forming a pseudo-3-fold symmetric framework.

Related Experiment Videos

  • A core beta-barrel structure with polar residues at the ends was identified.
  • A charged residue patch on the barrel surface is proposed as the IL-1 receptor binding site.

    • Conclusions:

    • The beta-barrel core of IL-1 is functionally important.
    • The identified binding surface provides insights into IL-1 receptor interactions.
    • Structural data aids in understanding IL-1's role in disease.