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Related Experiment Video

Updated: Mar 16, 2026

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
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Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

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Macromolecular Crystallography and Structural Biology Databases at NIST.

G L Gilliland1

  • 1Center for Advanced Research in Biotechnology of the University of Maryland Biotechnology Institute and the National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850.

Journal of Research of the National Institute of Standards and Technology
|August 9, 2016
PubMed
Summary
This summary is machine-generated.

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NIST advanced macromolecular crystallography using neutron and X-ray diffraction, contributing to structural biology and databases like the Protein Data Bank.

Area of Science:

  • Structural Biology
  • Macromolecular Crystallography
  • Biophysics

Background:

  • NIST initiated macromolecular crystallography in the late 1970s through collaboration with NIH.
  • Established capabilities in neutron and X-ray diffraction for crystal structure determination.
  • Biomolecular Structure Group formed in the mid-1980s, later joining the Center for Advanced Research in Biotechnology (CARB).

Purpose of the Study:

  • To detail the historical development and evolution of macromolecular crystallography and structural biology at NIST.
  • To highlight key research areas and collaborations over several decades.
  • To showcase NIST's contributions to structural biology databases and data management.

Main Methods:

  • Single-crystal neutron diffractometry.
Keywords:
macromolecular crystallographyneutron crystallographyprotein crystallographyproteinsstructural biology databasesx-ray crystallography

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  • Single-crystal X-ray diffraction.
  • Database development and management (Biological Macro-molecule Crystallization Database, Protein Data Bank).

    • Main Results:

    • Solved crystal structures of various proteins including bovine ribonuclease A, porcine insulin, chymosin, and subtilisin BPN'.
    • Advanced protein engineering studies and research on enzymes in amino acid biosynthesis pathways.
    • Played a crucial role in establishing and managing structural biology databases, including the Protein Data Bank.

    Conclusions:

    • NIST has a long-standing and significant history in macromolecular crystallography and structural biology.
    • Collaborative efforts have been key to advancing structural determination and data accessibility.
    • NIST continues to contribute to structural biology through research, data curation, and database stewardship.