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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Published on: January 17, 2020

Quantitative analysis of immobilized metalloenzymes by atomic absorption spectroscopy

Klaus Opwis1, Dierk Knittel, Eckhard Schollmeyer

  • 1Deutsches Textilforschungszentrum Nord-West e.V., Adlerstr. 1, 47798 Krefeld, Germany. opwis@dtnw.de

Analytical and Bioanalytical Chemistry
|November 13, 2004
PubMed

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Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
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Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
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