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Fluorometric sucrose evaluation for sugar beet.

Daniele Trebbi1, J Mitchell McGrath

  • 1Department of Crop and Soil Sciences, Plant Breeding and Genetics Graduate Program, Michigan State University, East Lansing, Michigan 48824-1325, USA.

Journal of Agricultural and Food Chemistry
|November 13, 2004
PubMed
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A new enzymatic assay accurately quantifies sucrose in young sugar beet seedlings for breeding. This method is rapid and cost-effective for selecting high-sucrose, disease-resistant sugar beet (Beta vulgaris) varieties.

Area of Science:

  • Agricultural Science
  • Plant Breeding
  • Biochemistry

Background:

  • Sucrose is the primary economic product of sugar beet (Beta vulgaris).
  • Disease resistance often resides in low-sucrose sugar beet genotypes, necessitating sucrose content selection during gene introgression.
  • Efficient sucrose quantification methods are crucial for breeding programs aiming to combine disease resistance with high yield.

Purpose of the Study:

  • To evaluate a rapid, inexpensive enzymatic-fluorometric microtiter plate assay for sugar beet root sucrose quantification.
  • To assess the assay's utility for both greenhouse progeny testing and field-grown mother root evaluation.
  • To compare enzymatic-fluorometric assay results with High-Performance Liquid Chromatography (HPLC) and refractometry.

Main Methods:

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  • Sucrose content was measured in diverse sugar and table beet populations across developmental stages using HPLC as a reference.
  • Enzymatic-fluorometric and refractometric assays were performed on the same samples.
  • Correlation analyses (R²) were conducted between HPLC and the other two methods.

    • Main Results:

    • Sucrose content ranged from 0.213 to 2.416 mmol g⁻¹ dry matter.
    • Refractometry generally overestimated sucrose content compared to HPLC.
    • Enzymatic-fluorometric assays showed high correlation with HPLC for young, greenhouse-grown tissues (R² = 0.976) but lower correlation for older, field-grown roots (R² = 0.605).

    Conclusions:

    • The enzymatic-fluorometric assay is a reliable and cost-effective method for sucrose quantification in young sugar beet seedlings.
    • This assay is suitable for progeny testing in breeding programs focused on enhancing sucrose content in disease-resistant lines.
    • Further investigation is needed to understand the reduced correlation in older, field-grown roots.