Related Experiment Videos
Nuclear factor kappa B activation by NADPH oxidases.
Robert A Clark1, Anthony J Valente
1Department of Medicine, University of Texas Health Science Center and South Texas Veterans Health Care System, San Antonio, TX 78229-3900, USA. clarkra@uthscsa.edu
Mechanisms of Ageing and Development
|November 16, 2004
Summary
Reactive oxygen species (ROS) from NADPH oxidase activate NF-kappaB. This pathway, involving gp91(phox), may play a role in aging, as shown by biphasic expression patterns in rat liver.
Area of Science:
- Biochemistry
- Cell Biology
- Immunology
Background:
- Reactive oxygen species (ROS) are key signaling molecules.
- NADPH oxidase is a primary source of ROS in phagocytes.
- NF-kappaB is a critical transcription factor activated by ROS.
Purpose of the Study:
- To investigate if NADPH oxidase products activate NF-kappaB.
- To explore the role of NADPH oxidase components in non-phagocytic cells.
- To examine the potential link between NADPH oxidase, NF-kappaB, and aging.
Main Methods:
- Transfection of K562 cells with NADPH oxidase components.
- Activation with phorbol ester, arachidonic acid, and TNFalpha.
- Gel shift and supershift assays to detect NF-kappaB activation and components.
Main Results:
- NADPH oxidase expression in K562 cells generated superoxide upon stimulation.
- NF-kappaB was induced by TNFalpha and phorbol ester in cells expressing NADPH oxidase components.
- gp91(phox) expression showed a biphasic pattern in aging rat liver.
Conclusions:
- NADPH oxidase products can induce NF-kappaB activation.
- The NADPH oxidase-NF-kappaB pathway is relevant in non-phagocytic cells.
- This pathway may contribute to the biological processes of aging.