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Immunohistochemical Staining of B7-H1 (PD-L1) on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue
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Immunohistochemistry and in situ hybridization in pancreatic neoplasia

Robb E Wilentz1, Ayman Rahman, Pedram Argani

  • 1Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Molecular Medicine
|November 16, 2004
PubMed

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...