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Serial analyses of gene expression (SAGE).

Jia Le Dai1

  • 1Department of Molecular Pathology, M.D. Anderson Cancer Center, University of Texas, Houston, USA.

Methods in Molecular Medicine
|November 16, 2004
PubMed
Summary

Serial analysis of gene expression (SAGE) is a molecular biology method for measuring global gene expression. This chapter details SAGE library preparation for transcriptome characterization and disease tissue comparison.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics

Background:

  • Serial analysis of gene expression (SAGE) is a technique to measure global gene expression levels.
  • SAGE has been successfully used to characterize transcriptomes.
  • It aids in comparing transcript levels between normal and diseased tissues and discovering novel molecules in signal transduction pathways.

Purpose of the Study:

  • To provide a detailed description of the procedures for preparing SAGE libraries.
  • To serve as a guide for researchers utilizing SAGE technology.

Main Methods:

  • Detailed protocols for SAGE library preparation are presented.
  • Focus is on the library preparation steps, excluding automated sequencing and general molecular biology techniques.

Main Results:

  • The chapter outlines the necessary steps for successful SAGE library construction.
  • It enables the generation of data for global gene expression analysis.

Conclusions:

  • The provided methods facilitate the preparation of SAGE libraries for comprehensive gene expression studies.
  • This chapter serves as a foundational resource for SAGE library preparation.

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