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Related Experiment Videos

Visualizing dendritic cell networks in vivo.

Randall L Lindquist1, Guy Shakhar, Diana Dudziak

  • 1Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA.

Nature Immunology
|November 16, 2004
PubMed
Summary

Dendritic cells (DCs) in lymph nodes maintain T cell tolerance. Mature DCs become motile, integrate into the lymph node network, and interact with T cells to initiate immune responses.

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Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Dendritic cells (DCs) are crucial for initiating immune responses and maintaining self-tolerance.
  • Tissue DCs mature upon innate immune signals, migrate to lymph nodes, and activate T cells.

Purpose of the Study:

  • To visualize dendritic cell behavior in vivo within lymph nodes.
  • To understand the dynamic interactions between DCs and T cells in the steady state and upon maturation.

Main Methods:

  • Generation of transgenic mice with enhanced yellow fluorescent protein-expressing DCs.
  • In vivo imaging using two-photon microscopy of live mouse lymph nodes.

Main Results:

  • Steady-state DCs form an extensive, stationary network, actively extending processes to interact with T cells.

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  • Mature DCs exhibit increased motility, dispersing and integrating into the existing DC network.
  • Enhanced DC motility facilitates interactions with migrating T cells.

    • Conclusions:

    • Lymph node DC network structure and dynamics are critical for regulating T cell interactions.
    • DC maturation alters their motility, influencing their integration and function within the lymph node microenvironment.
    • In vivo imaging provides insights into DC-T cell interactions governing immune responses and tolerance.