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Multi-Parameter Spectral Flow Cytometry Panel for Immune Phenotyping of Murine B and T Cell Responses

Kassandra Hoetzel1,2, Hendrik Feuerstein1,2, Julia Ludwig1

  • 1B Cell Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

European Journal of Immunology
|April 3, 2026
PubMed

To enable the analysis of B cell and T cell immune responses in mouse lymph nodes, spleen, and bone marrow, including different B cell and T cell subsets and their activation status, as well as the antigen-reactivity and isotype of B cells, a novel 31-parameter spectral flow cytometry panel was developed.

Keywords:
B cellsT cellsspectral flow cytometryvaccination

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