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Updated: Apr 4, 2026
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Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
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Multi-Parameter Spectral Flow Cytometry Panel for Immune Phenotyping of Murine B and T Cell Responses
Kassandra Hoetzel1,2, Hendrik Feuerstein1,2, Julia Ludwig1
1B Cell Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
European Journal of Immunology
|April 3, 2026
Summary
A new 31-parameter spectral flow cytometry panel allows detailed analysis of mouse immune cells. This method enhances the study of B cell and T cell immune responses and subsets in various tissues.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Understanding adaptive immunity requires detailed characterization of lymphocyte populations.
- Current methods may have limitations in simultaneously analyzing multiple immune cell subsets and their functions.
Purpose of the Study:
- To develop a comprehensive spectral flow cytometry panel for analyzing mouse immune responses.
- To enable deep phenotyping of B cell and T cell subsets.
Main Methods:
- Development of a novel 31-parameter spectral flow cytometry panel.
- Application of the panel to analyze immune cells from mouse lymph nodes, spleen, and bone marrow.
Main Results:
- The panel facilitates the analysis of diverse B cell and T cell subsets.
- It allows assessment of immune cell activation status and antigen-reactivity.
- B cell isotype analysis is also enabled.
Conclusions:
- The developed spectral flow cytometry panel is a powerful tool for immunological research.
- It provides a high-dimensional approach to dissecting complex immune responses in mice.
