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Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA)
Published on: December 19, 2019
Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
Alfredo Carlos S D Barros1, Elisa Naomi K Muranaka, Lincon Jo Mori
1Discipline of Gynecology, Hospital das Clínicas, Faculty of Medicine, University of São Paulo - São Paulo/SP, Brazil.
Purpose:
To test an experimental model of chemical mammary carcinogenesis induction in rats.
Methods:
Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy.
Results:
Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis.
Conclusion:
This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.
