Related Experiment Video
Updated: Aug 20, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Latex-functionalized monolithic columns for the separation of carbohydrates by micro anion-exchange chromatography
Emily F Hilder1, Frantisek Svec, Jean M J Fréchet
1E.O. Lawrence Berkeley National Laboratory, Materials Sciences Division, Berkeley, CA 94720, USA.
Abstract:
A novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2-acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch.
Related Concept Videos
Ion-Exchange Chromatography
Ion Exchange
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Affinity Chromatography

