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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Published on: March 6, 2009

Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes in an

Keiichi Kontani1, Koji Teramoto, Yoshitomo Ozaki

  • 1Department of Surgery, Shiga University of Medical Science, Seta-tsukinowa, Otsu 520-2192, Japan. konbat@med.kagawa-u.ac.jp

International Journal of Oncology
|November 18, 2004
PubMed

Insights

Researchers identified a new lung cancer immunotherapy target. A specific peptide derived from 90K/Mac-2 binding protein (M2BP) effectively stimulated cytotoxic T lymphocytes (CTLs) in HLA-A24 positive individuals, showing potential for cancer treatment.

Area of Science:

  • Immunology
  • Oncology
  • Molecular Biology

Background:

  • 90K/Mac-2 binding protein (M2BP) is highly expressed in lung cancer.
  • M2BP-specific immunity is observed in many lung cancer patients, suggesting its potential as a therapeutic target.
  • Previous findings indicated M2BP as a potential target antigen for cancer immunotherapy.

Purpose of the Study:

  • To select M2BP-derived peptides with an HLA-A24 binding motif.
  • To analyze the ability of these peptides to induce M2BP-specific cytotoxic T lymphocytes (CTLs).
  • To identify a novel M2BP-derived CTL epitope for lung cancer immunotherapy.

Main Methods:

  • Selected 11 peptides from M2BP with an HLA-A24 binding motif.
  • Generated CTLs using M2BP-derived peptides from CD8-positive T lymphocytes of HLA-A24-positive healthy donors.
  • Assessed CTL response via interferon-gamma production and reactivity against peptide-pulsed cells and cancer cells (TISI and BT20).

Main Results:

  • Two CTLs, induced by M2BP(241-250) and M2BP(568-576), produced interferon-gamma.
  • CTLs induced with M2BP(241-250) reacted with both peptide-pulsed TISI cells and M2BP/HLA-A24 expressing BT20 cells.
  • M2BP(241-250) was identified as a naturally processed epitope recognized by M2BP-specific CTLs in an HLA-A24 restricted manner.

Conclusions:

  • M2BP(241-250) is a naturally processed epitope derived from M2BP in cancer cells.
  • This M2BP-derived peptide is recognized by M2BP-specific CTLs in an HLA-A24 restricted manner.
  • The identified M2BP(241-250) CTL epitope is a promising target for clinical immunotherapy in lung cancer.

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