Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An extracellular driving force of cell-shape changes.

Otto Schmidt1, Ulrich Theopold

  • 1Insect Molecular Biology, Faculty of Sciences, University of Adelaide, Glen Osmond, SA 5064 Australia. otto.schmidt@adelaide.edu.au

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|November 20, 2004
PubMed
Summary

Cellular internalisation mechanisms, crucial for all organisms, remain poorly understood. This study proposes a novel

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell sequencing of tumor-associated macrophages in a <i>Drosophila</i> model.

Frontiers in immunology·2023
Same author

Chitinase-like proteins promoting tumorigenesis through disruption of cell polarity <i>via</i> enlarged endosomal vesicles.

Frontiers in oncology·2023
Same author

TRP channels, the missing link for Ca<sup>2+</sup> tuning by a unicellular eukaryotic parasite?

Cell calcium·2021
Same author

Anti-Fibrotic Activity of an Antimicrobial Peptide in a Drosophila Model.

Journal of innate immunity·2021
Same author

A Population Genomic Investigation of Immune Cell Diversity and Phagocytic Capacity in a Butterfly.

Genes·2021
Same author

Tissue-autonomous immune response regulates stress signaling during hypertrophy.

eLife·2020

Area of Science:

  • Cell Biology
  • Biophysics
  • Mechanobiology

Background:

  • Cellular internalisation, encompassing attachment, engulfment, and uptake, is a fundamental biological process observed across nearly all organisms.
  • While many internalisation reactions are linked to cell signalling pathways, the precise mechanical forces driving the formation of endocytotic vesicles are not yet elucidated.

Purpose of the Study:

  • To elucidate the unknown mechanical forces responsible for the formation of endocytotic vesicles during cellular internalisation.
  • To propose a novel mechanism that explains how cells generate the necessary forces for membrane invagination.

Main Methods:

  • Theoretical modeling of cellular membrane dynamics.
  • Analysis of lateral cross-linking processes on the cell surface.

Related Experiment Videos

  • Investigating the generation of configurational energy for membrane curvature.

    • Main Results:

    • A 'leverage-mediated' uptake mechanism is proposed as the driving force for endocytotic vesicle formation.
    • This mechanism involves lateral cross-linking processes on the cell surface.
    • These processes generate the configurational energy required to induce inverse membrane curvature.

    Conclusions:

    • The proposed leverage-mediated mechanism offers a new perspective on the biophysics of cellular internalisation.
    • Understanding these mechanical forces is critical for comprehending fundamental cellular processes and potential therapeutic interventions.
    • Further experimental validation is warranted to confirm the proposed mechanism in various biological contexts.