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[Analysis on intraspecific variation of Cnidium monnieri by RAPD]
Qiaoyan Zhang1, Luping Qin, Hanchen Zheng
1College of Pharmacy, Second Military Medical University, Shanghai 200433.
Objective:
To study the genetic variation of Cnidium monnieri from different regions.
Methods:
Random amplified polymophic DNA technique was used to analyze genetic polymorphy of Cnidium monnieri from 9 different regions, and dendrogram was constructed by UPGMA.
Results:
20 random primers were used for polymorphic selection. A total of 75 bands were amplified, 64 of which were polymorphism.
Conclusion:
The smaller the geographic distances between two Cnidium monnieri, the smaller genetic differences. However, it should not be overlooked that natural environment, especially the local ecological conditions may also show some influences on the genetic variations among different C. monnieri samples.