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[In vitro effect of interferon alpha on proliferation of human marrow fibroblast]
1Department of Hematology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, China.
Objective:
To explore the effect of interferon alpha (IFN-alpha) on proliferation of human marrow fibroblasts.
Methods:
Marrow fibroblasts were isolated from patients with iron deficiency anemia. Platelet derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1) or INF-alpha2b was added in the cell cultures each alone or in combinations. Cell proliferation and cell cycle of fibroblasts were detected by MTT and flow cytometry, respectively.
Results:
PDGF-ABqa (1 approximately 50 ng/ml) or TGF-beta1 (0.01 approximately 1 ng/ml) could stimulate the proliferation of the fibroblasts, while PDGF-AB was more effective (P < 0.01). IFN-alpha2b partially suppressed PDGF-AB induced proliferation of fibroblasts,the inhibition rate was 7.62%. IFN-alpha2b and TGF-beta1 combination suppressed synergistically not only the proliferation of the fibroblasts (P < 0.01) but also the PDGF-AB induced-proliferation (P < 0.01) at S phase.
Conclusion:
IFN-alpha2b combined with TGF-beta1 could synergistically suppress the PDGF-AB induced proliferation of fibroblasts at S phase in vitro.
