Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated spatial drift correction for EFTEM image series.

Bernhard Schaffer1, Werner Grogger, Gerald Kothleitner

  • 1Research Institute for Electron Microscopy, Graz University of Technology, Steyrergasse 17, A-8010 Graz, Austria. bernhard.schaffer@felmi-zfe.at

Ultramicroscopy
|November 24, 2004
PubMed
Summary

Drift in energy-filtered transmission electron microscopy (EFTEM) images causes artifacts. A new statistical method, statistically determined spatial drift (SDSD), corrects this drift for artifact-free analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility and strategies for direct atomic force microscopy on standard transmission electron microscopy specimens.

Micron (Oxford, England : 1993)·2025
Same author

Dual-Functional 3D-Nanoprinted AFM Probes for Correlative Magnetic and Conductive Characterization.

Small methods·2025
Same author

Workflows for multimodal electron tomography using EELS and EDX and their application to a spinodally decomposed CuNiFe alloy.

Ultramicroscopy·2025
Same author

Visualization of Cellulose Structures with Cesium Labeling and Cryo-STEM.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Splicing dual-range EELS spectra: Identifying and correcting artefacts.

Ultramicroscopy·2025
Same author

Incorrectly Focused Neodymium:Yttrium-Aluminum-Garnet (Nd:YAG) Laser Beam Leads to Massive Destructive Effects in Small-Aperture (Pinhole) Intraocular Lenses.

Ophthalmology and therapy·2024

Area of Science:

  • Materials Science
  • Physics
  • Chemistry

Background:

  • Energy filtering transmission electron microscopy (EFTEM) is crucial for analyzing elemental distribution and sample thickness.
  • Image artifacts arise from spatial drift between sequential image acquisitions in EFTEM series.
  • Accurate alignment is essential for reliable data extraction from EFTEM images.

Purpose of the Study:

  • To address the challenge of spatial drift in energy-filtered transmission electron microscopy (EFTEM) data.
  • To develop and present a novel, automated drift correction method for EFTEM image series.
  • To improve the accuracy and efficiency of data analysis in EFTEM.

Main Methods:

  • Developed a new drift correction approach using a statistical treatment of image data.

Related Experiment Videos

  • Implemented the statistically determined spatial drift (SDSD) correction program.
  • Applied the SDSD program to a typical EFTEM series data block for performance evaluation.
  • Main Results:

    • The new statistically determined spatial drift (SDSD) method effectively corrects spatial drift in EFTEM images.
    • SDSD demonstrates improved performance compared to existing methods, reducing the need for manual adjustments.
    • Artifact-free elemental distribution and thickness maps can be generated with the SDSD correction.

    Conclusions:

    • The statistically determined spatial drift (SDSD) method offers a robust solution for drift correction in EFTEM.
    • This approach enhances the reliability and efficiency of quantitative analysis in EFTEM.
    • SDSD facilitates more complex data analysis from EFTEM series by ensuring accurate image alignment.