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Updated: Aug 9, 2026

Electricity-Free, Sequential Nucleic Acid and Protein Isolation
Published on: May 15, 2012
Simple and rapid procedure suitable for quantitative isolation of low and high molecular weight extracellular nucleic
Svetlana N Tamkovich1, Pavel P Laktionov, Elena Yu Rykova
1Institute of Bioorganic Chemistry of Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Abstract:
The procedure based on binding of nucleic acids with glass surface in presence of chaotropic salts was adapted for efficient isolation of 100-10000 b.p. DNA fragments and 50-10,000 b. RNA fragments. The method provide 90% and 85% efficacy of isolation of 100 b.p. DNA and 100 b. RNA fragments respectively. High molecular weight nucleic acids are isolated with 98% efficacy. Isolated nucleic acids are free from contaminations, influencing nucleic acids modifying enzymes and fluorochromes. The method is rapid, simple and cost-effective.
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