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Updated: Aug 4, 2026
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
Published on: June 12, 2014
Comparison of PCR and antigen detection methods for diagnosis of Entamoeba histolytica infection
S J Furrows1, A H Moody, P L Chiodini
1Department of Microbiology, Cecil Fleming House, University College London Hospital, Grafton Way, London WC1H, UK. sfurrows@aol.com
Aims:
To assess different laboratory methods for the identification of Entamoeba histolytica in clinical samples.
Methods:
Antigen detection enzyme linked immunosorbent assay, polymerase chain reaction solution hybridisation enzyme linked immunoassay (PCR-SHELA), and a commercial Lightcycler PCR were compared using 101 stool and pus samples.
Results:
Fifteen of the 101 samples were positive for E histolytica by one or more method. There were discrepancies between the results in five of these 15 samples when the assays were compared.
Conclusions:
All three methods performed adequately, so that the choice of assay will depend on each individual laboratory's budget and projected turnaround time.
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