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Detection of prothrombin and osteopontin in a renal stone found in a hyperuricemic patient using 2D-PAGE and LC-MS
K Kaneko1, T Yamanobe, K Nakagomi
1Central Laboratory of Analytical Biochemistry, Faculty of Medicine, Teikyo University, Tokyo, Japan.
Abstract:
The liquid chromatography-mass spectrometry (LC-MS) following on from the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was applied for the analysis of proteins in a renal stone found in a hyperuricemic patient. This technique was sensitive enough to detect small quantities of proteins even in a renal stone.
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