Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Valproate decreases inositol biosynthesis.

Galit Shaltiel1, Alon Shamir, Joseph Shapiro

  • 1Stanley Research Center and Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Mental Health Center, Beersheva, Israel.

Biological Psychiatry
|December 4, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of CYP2C9 activity between Ethiopian and non-Ethiopian Jews: an interethnic study of (<i>S</i>)-warfarin pharmacokinetic and pharmacodynamic.

Frontiers in pharmacology·2026
Same author

Inositol biosynthesis is inversely regulated by glycolytic activity.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same author

Sex differences in animal models of complex disorders: Affective disorders, type 2 diabetes mellitus and essential hypertension.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Effects of acute fluctuations of extracellular Zinc on network activity of human iPSC-derived neurons; impact of NMDA Receptor and L-Type Calcium Channel Activation.

Neuropharmacology·2026
Same author

Does Administration of Low-Dose Aspirin Enhance the Efficacy of Psychotropic Drugs in Patients with Bipolar Disorder, Schizophrenia, and Schizoaffective Disorder?

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Human induced pluripotent stem cell-derived microglia with 1q21.1 deletion and duplication exhibit aberrant inflammatory response.

Genes & diseases·2026

Valproate (VPA) depletes inositol in the brain by inhibiting myo-inositol-1-phosphate (MIP) synthase. This finding suggests inositol depletion is a shared mechanism between VPA and lithium for treating bipolar disorder.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Lithium and valproate (VPA) are key treatments for bipolar disorder, yet their mood-stabilizing mechanisms remain unclear.
  • Inositol's role is increasingly recognized, with lithium known to inhibit inositol monophosphatase.
  • VPA's ability to deplete inositol in various models suggests a potential link to myo-inositol-1-phosphate (MIP) synthase.

Purpose of the Study:

  • To investigate whether VPA depletes inositol by inhibiting MIP synthase.
  • To determine if this effect is specific to VPA among psychotropic drugs.
  • To explore the impact of VPA and its derivatives on neuronal inositol levels and MIP synthase activity.

Main Methods:

  • Inositol levels were quantified using gas chromatography.

Related Experiment Videos

  • Human prefrontal cortex MIP synthase activity was measured.
  • Gene expression (INO1) and neuronal morphology were analyzed.

    • Main Results:

    • Acute VPA administration reduced inositol by 20% in mouse frontal cortex.
    • VPA significantly inhibited MIP synthase activity in a dose-dependent manner.
    • VPA specifically upregulated the yeast INO1 gene and affected neuronal morphology, unlike valpromide.

    Conclusions:

    • Valproate inhibits MIP synthase, the rate-limiting enzyme in inositol biosynthesis.
    • Inositol depletion is identified as a common initial mechanism for both VPA and lithium in bipolar disorder treatment.