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Related Concept Videos

What is Glycolysis?00:56

What is Glycolysis?

Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: Jun 6, 2026

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

Inositol biosynthesis is inversely regulated by glycolytic activity.

Chisom J Onu1, Michael Adu1, Dania Jabbar1

  • 1Department of Biological Sciences, Wayne State University, Detroit, MI, USA.

Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids
|June 4, 2026
PubMed
Summary

Altered glycolytic activity regulates inositol synthesis in yeast. Increased glycolysis reduces inositol levels, while decreased glycolysis enhances it, revealing metabolic crosstalk.

Keywords:
Dihydroxyacetone phosphate (DHAP)Glucose-6-phosphate (G-6-P)GlycolysisINO1InositolMyo-inositol phosphate synthase (MIPS)

More Related Videos

Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

Related Experiment Videos

Last Updated: Jun 6, 2026

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

Area of Science:

  • Metabolic regulation
  • Cellular biochemistry
  • Yeast genetics

Background:

  • Inositol is vital for cell function, including membrane biogenesis and signaling.
  • Inositol homeostasis disruption is linked to human diseases.
  • In yeast, inositol synthesis from glucose-6-phosphate (G-6-P) is rate-limited by myo-inositol phosphate synthase (MIPS).

Purpose of the Study:

  • To test the hypothesis that inositol synthesis is regulated by glycolytic activity.
  • To investigate the metabolic crosstalk between glycolysis and inositol synthesis pathways.

Main Methods:

  • Genetic and pharmacological manipulation of glycolysis in yeast.
  • Quantification of inositol synthesis using liquid chromatography mass spectrometry with [U-13C]-glucose.
  • Measurement of INO1 mRNA and MIPS protein expression levels.

Main Results:

  • Increased glycolytic activity (in rho0 cells and potassium cyanide-treated cells) reduced inositol levels by 47.5% and 57.9%, respectively.
  • Elevated glycolysis also decreased INO1 mRNA and MIPS protein expression.
  • Decreased glycolytic activity (in HHK2 cells) increased inositol synthesis by 41.5%.

Conclusions:

  • Altered glycolytic activity is a key mechanism for regulating inositol synthesis.
  • This study demonstrates significant metabolic crosstalk between glycolysis and inositol biosynthesis pathways.
  • Understanding this regulation is crucial for addressing disorders linked to inositol homeostasis.