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Related Experiment Videos

Boyden chamber assay.

Hong-Chen Chen1

  • 1Department of Life Science, National Chung Hsing University, Taichung, Taiwan.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2004
PubMed
Summary
This summary is machine-generated.

The Boyden chamber assay measures cell migration by observing cells moving through a membrane. This method is specifically adapted for Madin-Darby canine kidney cells using a 48-well trans-well system.

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Area of Science:

  • Cell biology
  • Biophysical techniques

Background:

  • The Boyden chamber assay is a standard method for studying cell chemotaxis.
  • It involves two compartments separated by a microporous membrane.
  • Cells migrate from an upper to a lower compartment containing chemoattractants.

Purpose of the Study:

  • To describe a specific protocol for measuring cell migration.
  • To detail the use of a 48-well Boyden chamber for Madin-Darby canine kidney cells.
  • To provide a standardized method for chemotaxis analysis.

Main Methods:

  • Utilizing a Boyden chamber with two compartments separated by a microporous membrane.
  • Placing Madin-Darby canine kidney cells in the upper compartment.
  • Incubating cells to allow migration towards chemoattractants in the lower compartment.

Related Experiment Videos

  • Fixing, staining, and quantifying migrated cells on the membrane.

    • Main Results:

    • The assay quantifies the migratory capacity of Madin-Darby canine kidney cells.
    • The method allows for the determination of chemotactic responses.
    • Successful migration is visualized and counted after staining.

    Conclusions:

    • The described method provides a reliable way to measure Madin-Darby canine kidney cell migration.
    • The Boyden chamber assay, also known as a trans-well migration assay, is versatile.
    • This technique is crucial for understanding cell movement in biological contexts.