Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Wound-healing assay.

Luis G Rodriguez1, Xiaoyang Wu, Jun-Lin Guan

  • 1Department of Molecular Medicine, Cornell University College of Veterinary Medicine, Ithaca, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomimetic Hydrogel System Targeting S100A8 Centered Neuroimmune Crosstalk and Hypoxia Induced Neuronal Injury.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Oxytocin attenuates fear learning via enhancing somatostatin interneuron-mediated local GABAergic inhibition in the prelimbic cortex.

Science advances·2026
Same author

A proteomic atlas of organelle remodeling identifies lysosomal SNX3 as a regulator of Notch signaling in epidermal differentiation.

bioRxiv : the preprint server for biology·2026
Same author

Comparative Genomics Reveals Genetic Adaptations to Diving-Associated Foraging in Anseriformes.

Ecology and evolution·2026
Same author

METTL14-dependent m<sup>6</sup>A modification restrains interferon signaling to prevent myocarditis and dilated Cardiomyopathy.

bioRxiv : the preprint server for biology·2026
Same author

Mitochondria power the cell's recycling center.

Autophagy·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

The wound-healing assay is a simple in vitro method that mimics in vivo wound healing to study cell migration. This technique quantifies cell migration rates and is useful for examining cell-matrix and cell-cell interactions.

Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • The wound-healing assay is a foundational technique for investigating cell migration.
  • It provides an in vitro model that simulates in vivo wound healing processes.

Purpose of the Study:

  • To describe the wound-healing assay for studying directional cell migration.
  • To highlight its utility in analyzing cell-matrix and cell-cell interactions.

Main Methods:

  • Creating a "wound" in a cell monolayer.
  • Capturing images at regular intervals to monitor wound closure.
  • Quantifying cell migration rates by comparing images.

Main Results:

  • The assay effectively quantifies cell migration rates.
  • It is suitable for studying factors influencing cell movement.

Related Experiment Videos

Conclusions:

  • The wound-healing assay is a versatile and accessible method for cell migration research.
  • Variations exist to track individual cell movements at the wound edge.