Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell-scatter assay.

Hong-Chen Chen1

  • 1Department of Life Science, National Chung Hsing University, Taichung, Taiwan.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2004
PubMed
Summary
This summary is machine-generated.

The scatter assay measures epithelial cell dispersion, a process mimicking epithelial-mesenchymal transition. This method quantifies cell scattering induced by factors like hepatocyte growth factor.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CDK1-mediated phosphorylation of EPLIN is crucial for mitotic spindle positioning.

Communications biology·2026
Same author

Secretory autophagy mediates SLC16A3/MCT4-dependent lactate secretion to drive metastatic progression in triple-negative breast cancer.

Autophagy·2026
Same author

Perinuclear assembly of vimentin intermediate filaments induces cancer cell nuclear dysmorphia.

The Journal of biological chemistry·2024
Same author

AKT2-mediated nuclear deformation leads to genome instability during epithelial-mesenchymal transition.

iScience·2023
Same author

Loss of cell-cell adhesion triggers cell migration through Rac1-dependent ROS generation.

Life science alliance·2022
Same author

MicroRNA-210 repression facilitates advanced glycation end-product (AGE)-induced cardiac mitochondrial dysfunction and apoptosis via JNK activation.

Journal of cellular biochemistry·2021
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Area of Science:

  • Cell Biology
  • Developmental Biology

Background:

  • Cell scattering describes epithelial cell colony dispersion induced by soluble factors.
  • This process involves membrane ruffling, cell detachment, and acquisition of a fibroblast-like, motile phenotype.
  • Epithelial cell scattering shares characteristics with epithelial-mesenchymal transition (EMT).

Purpose of the Study:

  • To describe a method for measuring the scatter response of epithelial cells.
  • To utilize the scatter assay for studying epithelial-mesenchymal transition (EMT).
  • To detect factors that induce cell migratory behavior.

Main Methods:

  • The scatter assay quantifies the dispersion of epithelial cell colonies.
  • The assay involves observing morphological changes and cell migration.

Related Experiment Videos

  • Specifically detailed for Madin-Darby canine kidney cells stimulated with hepatocyte growth factor.

    • Main Results:

    • Epithelial cell scattering is initiated by membrane ruffling and centrifugal spreading.
    • Detached cells acquire a motile, fibroblast-like morphology.
    • The assay effectively measures the response to specific growth factors.

    Conclusions:

    • The scatter assay is a valuable tool for investigating epithelial-mesenchymal transition (EMT).
    • It aids in identifying substances that promote cell motility.
    • The described method provides a standardized approach for studying cell scattering.