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Potency testing of acellular pertussis vaccines
1Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
Vaccine
|January 1, 1992
Summary
Assessing acellular pertussis vaccines is challenging. A new assay shows that antibodies inhibiting Bordetella pertussis adhesion to cells correlate with protection against lung colonization in mice.
Area of Science:
- Immunology
- Vaccinology
- Microbiology
Background:
- Assessing acellular pertussis vaccines' efficacy is a significant challenge.
- Current mouse models, like intracerebral challenge tests and respiratory infection assays, have limitations for acellular vaccines.
- There is a need for reliable laboratory assays to determine the potency of acellular pertussis vaccines.
Purpose of the Study:
- To evaluate the applicability of in vitro and in vivo assays for potency testing of acellular pertussis vaccines.
- To explore the correlation between Bordetella pertussis antigen-specific antibodies and protection against bacterial lung colonization in mice.
- To identify potential alternative assays for acellular pertussis vaccine potency.
Main Methods:
- Investigated the ability of Bordetella pertussis antigens to protect mice against lung colonization.
- Measured murine antibodies that inhibit bacterial adhesion to Vero cells.
- Assessed the correlation between in vitro antibody-mediated inhibition of adhesion and in vivo protection against lung colonization.
- Considered the relevance of these in vitro and in vivo assays for acellular pertussis vaccine potency testing.
Main Results:
- The ability of Bordetella pertussis antigens to protect mice against lung colonization was found to correlate with their capacity to induce murine antibodies.
- These antibodies demonstrated the ability to inhibit the adhesion of Bordetella pertussis to Vero cells.
- This correlation suggests a potential mechanism for vaccine-induced protection relevant to laboratory assessment.
Conclusions:
- In vitro assays measuring antibody-mediated inhibition of bacterial adhesion show promise for potency testing of acellular pertussis vaccines.
- The correlation between adhesion inhibition and protection against lung colonization supports the use of such assays.
- Further investigation into the applicability of these assays is warranted for reliable acellular pertussis vaccine potency determination.