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Drosophila Bys is nuclear and shows dynamic tissue-specific expression during development
Mary J Stewart1, Erik K Nordquist
1Department of Biological Sciences, North Dakota State University, Fargo, ND 58105, USA. Mary.Stewart@ndsu.nodak.edu
Development Genes and Evolution
|December 8, 2004
Summary
The function of the Bys-like gene family is conserved across species. Drosophila Bys protein is found in the nucleus, suggesting a conserved role in ribosome biogenesis, similar to yeast Enp1.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- The bys-like gene family is evolutionarily conserved from yeast to humans.
- Human Bystin is implicated in cell adhesion during embryo implantation.
- Yeast Enp1, a bys-like protein, functions in nuclear pre-ribosomal RNA splicing and ribosome biogenesis.
Purpose of the Study:
- To investigate the function of the Drosophila melanogaster bys-like gene family member, Bys.
- To examine the expression patterns of bys mRNA and the cellular localization of Bys protein in Drosophila.
Main Methods:
- Analysis of bys mRNA expression patterns in Drosophila embryos and larval wing imaginal discs.
- Determination of Bys protein localization in Schneider line 2 (S2) cells using epitope tagging.
Main Results:
- Bys mRNA exhibits tissue-specific expression during embryonic gastrulation and in specific regions of the larval wing imaginal disc.
- Expression patterns suggest potential roles in cell-cell interactions and processes regulated by Myc.
- Drosophila Bys protein localizes to the nucleus in S2 cells.
Conclusions:
- Drosophila Bys mRNA expression patterns are consistent with roles in cell adhesion and potentially cell growth.
- Nuclear localization of Bys protein suggests conserved function with yeast Enp1 in ribosome biogenesis.